Microsatellite Locus OSU16D

Primer Sequences

LOCUS       OSU16D        252 bp DNA                    12-AUG-1996
DEFINITION  Astatoreochromis alluaudi.
ACCESSION  U66812
KEYWORDS   STS .
SOURCE      Lake Victoria african cichlid adult DNA.
  ORGANISM  Astatoreochromis alluaudi
            Animalia; Chordata; Vertebrata; Actinopterygii; Neopterygii;
            Osteichthyes; Euteleostei; Acanthopterygii; Percomorpha;
            Perciformes; Percoidei; Percoidea; Cichlidae.
REFERENCE   1  (bases 1-252)
  AUTHORS   Wu, L., Booton, G. C., Kaufman, L., Chandler, M., and Fuerst, P. A.
  TITLE     Use of DNA microsatellite loci to identify populations and species
            of Lake Victoria haplochromine cichlids.
  JOURNAL   Aquaculture Biotechnology Symposium Proceedings, International
            Congress on the Biology of Fishes, Physiology Section, American
            Fisheries Society 1 (1), 105-113 (1996)
  STANDARD  full automatic
COMMENT
            These data kindly submitted in computer readable form by:
            PAUL A FUERST
            MOLECULAR GENETICS
            THE OHIO STATE UNIVERSITY
            484 W. 12TH AVENUE
            COLUMBUS, OHIO 43210-1292
            USA
            Phone:  614-292-6403
            Email:  pfuerst@magnus.acs.ohio-state.edu
            Fax:    614-292-4466

FEATURES            LOCATION/QUALIFIERS
     misc_feature    123..192
                     /*tag=  a
                     /label= STS
                     /note= "STS SIZE: 187"
                     /citation= ([1])
     satellite       145..164
                     /*tag=  b
                     /rpt_type= tandem
                     /rpt_family= "GT"
                     /standard_name= "DINUCLEOTIDE MICROSATELLITE"
                     /note= "4 ALLELES (TOTAL) OBSERVED IN THREE POPULATIONS.
                     STS ALLELE SIZES RANGE FROM 72 - 78 BP."
  primer_bind     123..140
                     /*tag=  c
                     /standard_name= "UPSTREAM PRIMER"
                     /label= PRIMER-U
                     /note= "PRIMER U SEQUENCE: GGCGAATGGTGGGTCAAG"
     primer_bind     complement (176..192)
                     /*tag=  d
                     /standard_name= "DOWNSTREAM PRIMER"
                     /label= PRIMER-D
                     /note= "PRIMER D SEQUENCE: ATGTTGCTTGCCGCTGC"
BASE COUNT       85 a     39 c     77 g     51 t
ORIGIN
        1 gatcagagag ttcagaagaa agagtaaaat acaggcaagt ggaagaagaa gaatgcagag
       61 gtggcagaga gttgaggaaa gaaaagaaaa gaaacaaaaa ggacccgatg aggtgccctc
      121 cgggcgaatg gtgggtcaag aaaagtgtgt gtgtgtgtgt gtgtgcgttc acatggcagc
      181 ggcaagcaac atttacaaag aggcagctgt tccattagct actgcatttg tgtctaatta
      241 catcctggtc tc

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