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NEUROSCI 702 - INTRODUCTION TO TOPICS IN CELL BIOPHYSICS (3 CREDIT HOURS)
Michael X. Zhu, Course Director
Textbook: Molecular Cell Biology, Lodish, et al., Publisher: W. H. Freeman; 5th edition 2004
Website: http://carmen.osu.edu
Monday, Wednesday, and Friday: 11:30 am -12:18 pm
Course Objectives:
To develop basic knowledge of Cell Biophyscis, including structure and function of biological membranes,
intracellular organelles, cytoskeleton, cellular basis of electrical excitability, regulation of biomolecule
synthesis, signal transduction, biophysical properties of muscle fibers, and information processing in the nervous system.
Emphases are placed on understanding the biophysical aspects of cell physiology as well as the biophysical approaches used
in molecular and cellular physiology.
The course is purposefully organized for students with diverse background to gain fundamental knowledge of Cell Biophysics.
Student Evaluation:
Students will be given ten take home assignments with assay questions. The answers will be graded. The 8 assignments with
the highest scores will be included in the final grade (80%). Class participation (attendance and participation in class discussion)
will account for 20% of the final grade.
Course Schedule:
| Week # | Topic | Instructor |
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| 1 | Course Introduction: Overview of Current topics in Biophysics | Zhu |
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| Biophysical Properties of Cell Membranes | Parinandi |
| 2 | Molecular basis of membrane transport | Nair |
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| Structure and Dynamics of a Protein-conducting Membrane Channel | Dalbey |
| Origin of membrane potential in cells | Zhu |
| 3 | Generation of the Action Potential | Zhu |
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| Patch Clamping-Ca channel activity | Zhu |
| Lab Demo Patch Clamping | Zhu lab |
| 4 | Cytoskeleton overview | L. Moldovan |
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| Viscoelastic Properties of tissues/proteins, etc. | N. Moldovan |
| Encoding Neuronal Information | Mangel |
| 5 | Image Processing in the Retina | Mangel |
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| Introduction to Modeling Neuronal Behavior | Terman |
| Studying Mechanical Properties Atomic Force | Agarwal |
| 6 | Lab Demo Atomic Force Microscopy | Agarwal lab |
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| Overview of Molecular Motors | Davis |
| Overview of contractile proteins in skeletal and cardiac muscle | Tikunova |
| 7 | Lab Demo Measurements of single fiber contractile function | Rall lab |
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| Neurofilament Transport Mechanisms | Brown |
| Introduction Mitochondria | Ghafourifar |
| 8 | Regulation of Mitochondrial function/Role in cell signaling | Ghafourifar |
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| Introduction to Cell Signaling Networks | Tridandapani |
| Protein-protein interactions; the Inflammasome | Wewers |
| 9 | Overview of Calcium Signaling | Zhu |
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| Calcium Signaling and EC coupling in the heart | Ziolo |
| 10 | Biophysical approaches to studying quantum calcium events | Gyorke |
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| Lab Demo Calcium sparks/ Confocal Microscopy | Gyorke lab |
| 11 | Introduction to DNA/RNA and Protein Synthesis | Periasamy |
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| DNA/RNA and Protein Synthesis Continued | Periasamy |
| Regulation of chromatin condensation | Poirier |
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