Venkat Gopalan
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Associate Professor
Phone: 614-292-1332
Fax: 614-292-6773
email: gopalan.5@osu.edu
Webpage: Gopalan Homepage
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Research Interests:
Structure and mechanism of action of ribonuclease P, a catalytic
ribonucleoprotein (RNP) complex. The endoribonuclease RNase P
is a ubiquitous RNP that is involved in the maturation of tRNAs. In
Escherichia coli, the RNase P holoenzyme consists of a catalytic RNA
subunit (M1 RNA, 377 nts) and a protein cofactor (C5 protein, 119
amino acid residues). The long-term goal of our studies is to elucidate
the mechanism of assembly of this unique catalytic RNP complex and
thereby understand how an RNA-binding protein influences the structure
and function of its RNA ligand. 2. Use of plant RNase P to inhibit
gene expression Recently, it has been demonstrated that endogenous,
cellular RNase P can be guided to specifically destroy a target mRNA
in bacterial and human cells in tissue culture. Our aim is to determine
if plant RNase P could be utilized to disrupt gene expression in plants
and their pathogens. These studies will help establish plant RNase
P as a valuable functional genomics tool. Towards this objective,
we have recently made significant progress in partially purifying
and characterizing plant RNase P.
Publications
Selected publications from the last 5 years:
Gopalan, V.. (2007) "Uniformity amid diversity in RNase P." Proc Natl Acad Sci U S A. 104(7), 2031-2.
Tsai HY, Pulukkunat DK, Woznick WK, Gopalan, V.. (2006) "Functional reconstitution and characterization of Pyrococcus furiosus RNase P." Proc Natl Acad Sci U S A. 103(44), 16147-52.
Rangarajan S, Raj ML, Hernandez JM, Grotewold E, Gopalan
V. (2004) "RNase
P as a tool for disruption of gene expression in maize cells.", Biochem
J. 380(Pt 3), 611-6.
Pulukkunat DK, Raj ML, Pattanayak D, Lai LB, Gopalan
V. (2003) "Exploring
the potential of plant RNase P as a functional genomics tool.". Methods
Mol Biol. 236, 295-310.
Boomershine, W.P., Stephen Raj, M.L., Gopalan
V., and Foster, M.P.
(2003) "Preparation of uniformly labeled NMR samples in Escherichia
coli under the tight control of the araBAD promoter: expression
of an archaeal homolog of the RNase P Rpp29 protein." Protein
Expr Purif. 28, 246-51.
Tsai, H.Y., Masquida, B., Biswas, R., Westhof, E., and Gopalan
V. (2003) "Molecular modeling of the three-dimensional structure
of the bacterial RNase P holoenzyme." J Mol Biol. 325,
661-75.
Jovanovic, M., Sanchez, R., Altman, S., and Gopalan
V. (2002) "Elucidation
of structure-function relationships in the protein subunit of bacterial
RNase P using a genetic complementation approach." Nucleic
Acids Res.30, 5065-73.
Guerrier-Takada, C., Eder, P.S., Gopalan
V., and Altman, S. (2002) "Purification
and characterization of Rpp25, an RNA-binding protein subunit of human
ribonuclease P." RNA 8, 290-5.
Tsai, H.Y., Lai, L.B., and Gopalan V. (2002) "A Modified pBluescript-based
vector for facile cloning and transcription of RNAs." Anal
Biochem.303, 214-7.
Eubank, T.D., Biswas, R., Jovanovic, M., Litovchick, A., Lapidot, A.,
and Gopalan V. (2002) "Inhibition of bacterial RNase P by aminoglycoside-arginine
conjugates." FEBS Lett. 511, 107-12.
Gopalan, V., Vioque, A., and Altman, S. (2002) "RNase P: variations
and uses." J Biol Chem. 277, 6759-62.
Stephen Raj, M. L., Pulukkunat, D. K., Reckard, J. F., Thomas, G. and
Gopalan V. (2001.) "Cleavage of bipartite substrates by rice
and maize RNase P: Application to targeted degradation of mRNAs in plants." Plant
Physiology 125: 1187-1190.
Biswas, R., Kuhne, H., Brudvig, G. and Gopalan
V. (2001) "Use
of EPR spectroscopy to study macromolecular function." Science
Progress 84: 45-68.
Wu, C-W., Eder, P.S., Gopalan V. and Behrman, E. J. (2001). "Kinetics
of coupling reactions that generate monothiophosphate disulfides Implications
for modification of RNAs." Bioconjugate Chemistry 12: 842-844.
