Pictures from left to right: 1) Elkhorn Mts in Oregon above Anthony Lakes (fieldwork for Penstemon project); 2) Hybrid swarm of P. cardwellii and P. rupicola at Santiam Pass in Cascade Mts of Oregon; 3) Sympatric P. spectabilis and P. centranthifolius in San Diego Co., California; 4) Hyobanche glabrata pushing out from beneath a piece of shale in South Africa.
Photo credits: Andrea D. Wolfeİ
Systematics Research in the Wolfe Lab
My systematics interests are in tribal and subgeneric relationships of taxa within Scrophulariaceae. Systematics projects currently in progress include: 1) phylogenetic reconstruction of the genus Penstemon; 2) investigations of hybridization and introgression among species of Penstemon using microsatellite-based markers; 3) an investigation of tribe Cheloneae, and 4) systematics of the holoparasitic genus Hyobanche.
Student projects in the lab include systematics of Penstemon subgenus Dasanthera, systematics of the holoparasitic genus Harveya, molecular evolution and biogeography of Monotropaceae, systematics of Zalusianskya , and conservation biology of Penstemon caryi.
My lab is set-up for molecular systematics work. Routine protocols include DNA and RNA extraction; PCR; Southern blot, Northern blot, and dot blot methods; and DNA sequencing. Protein electrophoresis labs are set up nearby in Dan Crawford's area.
My current lab consists of two recently renovated rooms totaling about 750 sq. ft. of space. Equipment includes DNA sequencing and agarose gel rigs, power supplies, thermocyclers, gel dryer and vacuum pump, DNA hybridization oven, UV crosslinker, Genequant calculator, vacuum oven, water baths, heat blocks, vortexers, orbital rotators, a digital gel imaging system, balances, computer equipment, freezers and refrigerators, microcentrifuges, heating and nonheating stirplates, and access to ultra- and superspeed centrifuges. The Department of Evolution, Ecology and Organismal Biology has recently acquired an ABI 310 automated sequencer.
Supporting facilities include a new greenhouse facility and a large herbarium in the Museum of Biological Diversity.
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Last updated September 29, 1998.