Tris Borate EDTA 10X Powder
45451

        **** SECTION 1 - CHEMICAL PRODUCT AND COMPANY IDENTIFICATION ****

MSDS Name: Tris Borate EDTA 10X Powder
Catalog Numbers:
    BP1334 1, BP1334-1, BP13341
Synonyms:
    Tris-Boric Acid-ETDA Powder, TBE Powder
Company Identification:  Fisher Scientific
                         1 Reagent Lane
                         Fairlawn, NJ  07410
For information, call:  201-796-7100
Emergency Number:       201-796-7100
For CHEMTREC assistance, call: 800-424-9300
For International CHEMTREC assistance, call: 703-527-3887

         **** SECTION 2 - COMPOSITION, INFORMATION ON INGREDIENTS ****

+----------------+--------------------------------------+----------+-----------+
|      CAS#      |              Chemical Name           |    %     |  EINECS#  |
|----------------|--------------------------------------|----------|-----------|
|       60-00-4  |Ethylenediamine tetraacetic acid      |   3.5    | 200-449-4 |
|----------------|--------------------------------------|----------|-----------|
|       77-86-1  |Tris (hydroxymethyl) aminomethane     |    64    | 201-064-4 |
|----------------|--------------------------------------|----------|-----------|
|    10043-35-3  |Boric acid                            |   32.5   | 233-139-2 |
+----------------+--------------------------------------+----------+-----------+

                  **** SECTION 3 - HAZARDS IDENTIFICATION ****

                              EMERGENCY OVERVIEW
Appearance:  white.
Caution! May cause kidney damage. May cause central nervous system
effects. May cause reproductive effects based upon animal studies.
May cause eye and skin irritation. May cause respiratory and
digestive tract irritation.
Target Organs: Kidneys, central nervous system.

Potential Health Effects
    Eye:
         May cause eye irritation.
    Skin:
         May cause skin irritation.
    Ingestion:
         May cause irritation of the digestive tract. May cause kidney
         damage. May cause central nervous system effects.
    Inhalation:
         May cause respiratory tract irritation.
    Chronic:
         May cause borism characterized by dry skin, skin eruptions, and
         gastric disturbances..

                   **** SECTION 4 - FIRST AID MEASURES ****

    Eyes:
         Immediately flush eyes with plenty of water for at least 15 minutes,
         occasionally lifting the upper and lower eyelids. Get medical aid
         immediately.
    Skin:
         Get medical aid if irritation develops or persists. Flush skin with
         plenty of soap and water.
    Ingestion:
         If victim is conscious and alert, give 2-4 cupfuls of milk or water.
         Never give anything by mouth to an unconscious person. Get medical
         aid immediately.
    Inhalation:
         Remove from exposure to fresh air immediately. If not breathing,
         give artificial respiration. If breathing is difficult, give oxygen.
         Get medical aid.
    Notes to Physician:
         Treat symptomatically and

                 **** SECTION 5 - FIRE FIGHTING MEASURES ****

    General Information:
         As in any fire, wear a self-contained breathing apparatus in
         pressure-demand, MSHA/NIOSH (approved or equivalent), and full
         protective gear. During a fire, irritating and highly toxic gases
         may be generated by thermal decomposition or combustion.
    Extinguishing Media:
         Substance is noncombustible; use agent most appropriate to
         extinguish surrounding fire.

               **** SECTION 6 - ACCIDENTAL RELEASE MEASURES ****

    General Information: Use proper personal protective equipment as indicated
                         in Section 8.
    Spills/Leaks:
         Sweep up or absorb material, then place into a suitable clean, dry,
         closed container for disposal. Avoid generating dusty conditions.

                  **** SECTION 7 - HANDLING and STORAGE ****

    Handling:
         Wash thoroughly after handling. Use with adequate ventilation.
         Minimize dust generation and accumulation. Avoid contact with eyes,
         skin, and clothing. Keep container tightly closed. Avoid ingestion
         and inhalation.
    Storage:
         Store in a tightly closed container. Store in a cool, dry,
         well-ventilated area away from incompatible substances. Keep
         containers tightly closed.

         **** SECTION 8 - EXPOSURE CONTROLS, PERSONAL PROTECTION ****

    Engineering Controls:
         Use adequate ventilation to keep airborne concentrations low.

                                Exposure Limits
+--------------------+-------------------+-------------------+-----------------+
|   Chemical Name    |        ACGIH      |       NIOSH       |OSHA - Final PELs|
|--------------------|-------------------|-------------------|-----------------|
| Ethylenediamine tet|none listed        |none listed        |none listed      |
| raacetic acid      |                   |                   |                 |
|--------------------|-------------------|-------------------|-----------------|
| Tris (hydroxymethyl|none listed        |none listed        |none listed      |
| ) aminomethane     |                   |                   |                 |
|--------------------|-------------------|-------------------|-----------------|
| Boric acid         |none listed        |none listed        |none listed      |
+--------------------+-------------------+-------------------+-----------------+

    OSHA Vacated PELs:
         Ethylenediamine tetraacetic acid:
            No OSHA Vacated PELs are listed for this chemical.
         Tris (hydroxymethyl) aminomethane:
            No OSHA Vacated PELs are listed for this chemical.
         Boric acid:
            No OSHA Vacated PELs are listed for this chemical.

    Personal Protective Equipment

                Eyes:
                      Wear appropriate protective eyeglasses or chemical
                      safety goggles as described by OSHA's eye and face
                      protection regulations in 29 CFR 1910.133 or European
                      Standard EN166.
                Skin:
                      Wear appropriate protective gloves to prevent skin
                      exposure.
            Clothing:
                      Wear appropriate protective clothing to prevent skin
                      exposure.
         Respirators:
                      Follow the OSHA respirator regulations found in 29CFR
                       1910.134 or European Standard EN 149. Always use a
                       NIOSH or European Standard EN 149 approved respirator
                      when necessary.

            **** SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES ****

Physical State:             Solid
Appearance:                 white
Odor:                       odorless
pH:                         Not available.
Vapor Pressure:             Not available.
Vapor Density:              Not available.
Evaporation Rate:           Not available.
Viscosity:                  Not available.
Boiling Point:              Not available.
Freezing/Melting Point:     Not available.
Autoignition Temperature:   Not applicable.
Flash Point:                Not applicable.
NFPA Rating:                Not published.
Explosion Limits, Lower:    Not available.
                 Upper:    Not available.
Decomposition Temperature:  Not available.
Solubility:                 Soluble in water.
Specific Gravity/Density:   Not available.
Molecular Formula:          Mixture
Molecular Weight:           0

                **** SECTION 10 - STABILITY AND REACTIVITY ****

    Chemical Stability:
         Stable at room temperature in closed containers under normal storage
         and handling conditions. Decarboxylates above 150C.
    Conditions to Avoid:
         Incompatible materials.
    Incompatibilities with Other Materials:
         EDTA is incompatible with: strong oxidizing agents, strong bases,
         copper, copper alloys, aluminum. Tris is incompatible with strong
         oxidizing agents and strong acids. Boric acid is incompatible with
         acetic anhydride and potassium. Forms borate salts with basic
         compounds.
    Hazardous Decomposition Products:
         Carbon monoxide, carbon dioxide, nitric oxide (NOx) and ammonia (NH3)
         fumes.
    Hazardous Polymerization: Has not been reported.

               **** SECTION 11 - TOXICOLOGICAL INFORMATION ****

    RTECS#:
         CAS# 60-00-4: AH4025000
         CAS# 77-86-1: TY2900000
         CAS# 10043-35-3: ED4550000
    LD50/LC50:
         CAS# 60-00-4: Oral, mouse: LD50 = 30 mg/kg.
         CAS# 77-86-1: Oral, rat: LD50 = 5900 mg/kg.
         CAS# 10043-35-3: Oral, mouse: LD50 = 3450 mg/kg; Oral, rat: LD50 =
          2660 mg/kg.
     Carcinogenicity:
      Ethylenediamine tetraacetic acid -
         Not listed by ACGIH, IARC, NIOSH, NTP, or OSHA.
      Tris (hydroxymethyl) aminomethane -
         Not listed by ACGIH, IARC, NIOSH, NTP, or OSHA.
      Boric acid -
         Not listed by ACGIH, IARC, NIOSH, NTP, or OSHA.
    Epidemiology:
         No information available.
    Teratogenicity:
         EDTA: Embryo or Fetus, Stunted fetus, oral-rat TDLo=7632mg/kg.
         Specific Developmental Abnormalities, Cardiovascular, Craniofacial,
         Musculoskeletal, Respiratory, and Urogenital, oral-rat
         TDLo=7632mg/kg. Boric Acid: Decreased fetal weight, oral rat 13.3
         mg/kg/1D.
    Reproductive Effects:
         EDTA: Fertility, Post-implantation mortality, oral-rat
         TDLo=7632mg/kg. Boric Acid: Maternal effects on ovaries and
         fallopian tubes, oral rat TDLo=45 g/kg 90 days. Paternal effects on
         spermatogenesis, oral rat TDLo=52 mg/kg. effects on testes, sperm
         duct or epididymis, oral rat TDLo=45 g/kg 90 days.
    Neurotoxicity:
         No information available.
    Mutagenicity:
         EDTA: Cytogenetic Analysis, intraperitoneal-mouse 50mmol/L. DNA
         Inhibition: hamster fibroblast 500ug/L, rabbit kidney 250umol/L.
    Other Studies:
         None.

                 **** SECTION 12 - ECOLOGICAL INFORMATION ****

    Ecotoxicity:
         EDTA: Catfish (tap water) 129ppm/96H.

                **** SECTION 13 - DISPOSAL CONSIDERATIONS ****

Chemical waste generators must determine whether a discarded chemical is classif
as a hazardous waste.
US EPA guidelines for the classification determination are listed in 40 CFR Part
Additionally, waste generators must consult state and local hazardous waste regu
ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series: None listed.

                 **** SECTION 14 - TRANSPORT INFORMATION ****

    US DOT
         No information available
    Canadian TDG
         No information available.

                 **** SECTION 15 - REGULATORY INFORMATION ****

US FEDERAL
    TSCA
         CAS# 60-00-4 is listed on the TSCA inventory.
         CAS# 77-86-1 is listed on the TSCA inventory.
         CAS# 10043-35-3 is listed on the TSCA inventory.
       Health & Safety Reporting List
         None of the chemicals are on the Health & Safety Reporting List.
       Chemical Test Rules
         None of the chemicals in this product are under a Chemical Test Rule.
       Section 12b
         None of the chemicals are listed under TSCA Section 12b.
       TSCA Significant New Use Rule
         None of the chemicals in this material have a SNUR under TSCA.
    SARA
       Section 302 (RQ)
         CAS# 60-00-4: final RQ = 5000 pounds (2270 kg)
       Section 302 (TPQ)
         None of the chemicals in this product have a TPQ.
       SARA Codes
         CAS # 77-86-1: acute.
         CAS # 10043-35-3: acute.
       Section 313
         No chemicals are reportable under Section 313.
    Clean Air Act:
         This material does not contain any hazardous air pollutants.
         This material does not contain any Class 1 Ozone depletors.
         This material does not contain any Class 2 Ozone depletors.
    Clean Water Act:
         CAS# 60-00-4 is listed as a Hazardous Substance under the CWA.
         None of the chemicals in this product are listed as Priority
         Pollutants under the CWA.
         None of the chemicals in this product are listed as Toxic Pollutants
         under the CWA.
    OSHA:
         None of the chemicals in this product are considered highly hazardous
         by OSHA.
STATE
    Ethylenediamine tetraacetic ac can be found on the following state
    right to know lists: California, New Jersey, Pennsylvania,
    Massachusetts.
    Tris (hydroxymethyl) aminomethane is not present on state lists from
    CA, PA, MN, MA, FL, or NJ.
    Boric acid is not present on state lists from CA, PA, MN, MA, FL, or
    NJ.
    California No Significant Risk Level:
    None of the chemicals in this product are listed.
European/International Regulations
    European Labeling in Accordance with EC Directives
         Hazard Symbols: Not available.
         Risk Phrases:
         Safety Phrases:
  WGK (Water Danger/Protection)
         CAS# 60-00-4: 2
         CAS# 77-86-1: 1
         CAS# 10043-35-3: 1
  United Kingdom Occupational Exposure Limits

  Canada
         CAS# 60-00-4 is listed on Canada's DSL/NDSL List.
         CAS# 77-86-1 is listed on Canada's DSL/NDSL List.
         CAS# 10043-35-3 is listed on Canada's DSL/NDSL List.
         WHMIS: Not available.
         CAS# 60-00-4 is not listed on Canada's Ingredient Disclosure List.
         CAS# 77-86-1 is not listed on Canada's Ingredient Disclosure List.
         CAS# 10043-35-3 is not listed on Canada's Ingredient Disclosure List.
  Exposure Limits
         CAS# 10043-35-3: OEL-RUSSIA:STEL 10 mg/m3

                 **** SECTION 16 - ADDITIONAL INFORMATION ****

    MSDS Creation Date:  3/24/1998  Revision #2 Date:  8/02/2000

    The information above is believed to be accurate and represents the best
    information currently available to us. However, we make no warranty of
    merchantability or any other warranty, express or implied, with respect to
    such information, and we assume no liability resulting from its use. Users
    should make their own investigations to determine the suitability of the
    information for their particular purposes. In no way shall the company be
    liable for any claims, losses, or damages of any third party or for lost
    profits or any special, indirect, incidental, consequential or exemplary
    damages, howsoever arising, even if the company has been advised of
    the possibility of such damages.
--------------------------------------------------------------------------------