Services - Proteomics
Sample Results

Example of proteins separated by 2-dimensional electrophoresis and identification of a single spot by peptide fingerprinting.

Figures A and B are soluble protein extracts from wildtype and mutant Chlorobium tepidum respectively. The mutant strain was made by knocking out the rubisco-like protein (omega::RLP) by insertional inactivation. Figure C is an expanded view of the yellow regions in A and B, and the circled protein spot was cored for identification by mass spectrometry. Figure D shows the sizes of the peptide fragments after trypsin digestion which agree with the predicted sizes for a thiol-specific antioxidase gene (tsaA). The TSA protein level is approximately 10 fold higher in the mutant strain which agrees with the mRNA levels. The results are courtesy of Tom Hanson, Ph.D. (Dept.of Microbiology).

Figure A Figure B Figure C Figure D
Figure A
Wildtype (wt)
Figure B
Omega::RLP
Figure C
Expanded view of yellow regions from Figs A and B
Figure D
Mass spectrometry data for circled spot in Fig C

Note: The trypsin digestion and subsequent mass spectrometry analysis was done at the CCIC-Mass Spectrometry Facility at OSU.