Services - DNA Sequencing Electropherogram
Figure 8
Electropherograms that have homoN slippage.

The sequence is characterized by a stretch of 5 or more “As”, “Ts”, “Gs”, or “Cs” that results in poor sequence three prime of this homoN area. The problem is caused by the DNA separating and reannealing incorrectly at either base +1, -1, +2, -2, etc. resulting in a distribution of peaks around each base. A mild case homoT produces a few “N”s (a) whereas a severe case of homoT results in the sequence appearing as waves (b). The best solution is to sequence the complementary strand in this region.



 Fig. 8a, Electropherograms that have homoN slippage.


Fig. 8b, Electropherograms that have homoN slippage.