Services - DNA Sequencing Electropherogram
Figure 7
Electropherogram that has multiple sequences.

The electropherogram demonstrates multiple peaks for each position, and the peaks are in phase with each other. For example, base 34 has a “T” peak under the “G” peak. This problem can be due to multiple templates, multiple priming sites or multiple primers. Below is a special case, multiple inserts, in which there are two different plasmids present that share the same vector (bases 1 – 33), but they have different inserts (bases 34 - 160). The solution is to separate the plasmids and resequence, e.g. restreaking the bacterial culture that contains the plasmids and then purify the plasmid again.



 Fig. 7a, Electropherogram that has multiple sequences.


Fig. 7b, Electropherogram that has multiple sequences.