Services - DNA Sequencing Electropherogram
Figure 6
Electropherogram that demonstrates a lack of extension products, i. e. no bands.

Large initial peaks from the unincorporated nucleotides followed by a nearly flat line with all 4 colors mixed together characterize the electropherogram below. The causes of this result are numerous including template or primer that is: low quality, low concentration, or degraded. Another cause could be the lack the primer binding site. Also, this may be the result of a simple error on the part of the operator, such as not adding a reagent to the reaction, or a malfunction by the 3730 DNA Analyzer, such as an air bubble in the transfer syringe. The solutions are as varied as the problems described above, e.g. repeating the reaction, purifying the template again or redesigning the primer.



 Fig. 6a, Electropherogram that demonstrates a lack of extension products, i. e. no bands.


Fig. 6b, Electropherogram that demonstrates a lack of extension products, i. e. no bands.