Services - DNA Sequencing Electropherogram
Figure 5
Electropherogram with mobility errors.

Mobility errors only occur in the first 100 bases, and are characterized by peaks that are to close together or overlapping. This problem is sequence dependent and caused by limitations in the software’s inability to accurately judge the spacing between the peaks in the electropherogram. For example, bases 10 through 16 are “CTNNCT” due to overlap of the peaks, but the sequence is actually “CTCACT”. Also, the “N”s at base 47 and 56 should not be there, but the software is expecting a peak since the previous peaks are shifted to the left. The best solution is to edit the sequence manually.



 Electropherogram with mobility errors.