Services - DNA Sequencing Electropherogram
Figure 3
Electropherogram that demonstrates the limit of the resolution of the 3730 DNA Analyzer.

The electropherogram below demonstrates how the bands for the extension products eventually become too wide for proper interpretation by the sequencing analysis software. When the width of the base of the peak begins to approach 1/4 of the peak height, then resolution maybe lost. Even though the absolute sequence is not always correct the sequence is indicative of the bases present. For example the “T”s (underline and lower case) at 782 and 830 were called as “N”s by the sequencing program. The program often times inserts bases to accommodate a broad peak, for example the sequence from 801 to 810 should be CTTCTGAG and the sequence from 815 to 820 should be AGTGG. The best solution for this limitation is to manually edit the sequence.



 Electropherogram that demonstrates the limit of the resolution of the 3730 DNA Analyzer.