Services - DNA Sequencing Electropherogram
Figure 12
Electropherogram that has Primer N-1.

The sequence is characterized by having multiple peaks at all of the locations and these peaks are arranged such that the same sequence is present but shifted by one, two or more positions. For example, the “C” at position 289 is represented by a smaller peak at base 288 and an even smaller peak at base 287. This problem is caused by a significant percentage of the primers being short by 1, 2 or more bases. For example, the primer for the reactions below was 20 bases in length, but many molecules are 19 bases long and a few are 18 bases long. The best solution to the problem is to use another primer and do another sequencing reaction. Another solution is to purify the primer, or order a primer that is purified followed by repeating the sequencing reaction.


 Electropherogram that has Primer N-1