Biochemistry Faculty
Zhengrong (Justin) Wu
Assistant Professor
Phone: 614-247-5040
Fax: 614-292-6773
email: wu.473@osu.edu
Webpage: None
Research Interests:
Macromolecular interaction, including protein-protein and protein-nucleic
acid plays critical roles in almost every signaling pathway. Recent
development of residual dipolar couplings (RDCs) in modem NMR spectroscopy
has extended its speed, accuracy and scope of structure determination.
Studies of these interactions by NMR may provide useful insight into
the function and mechanism of those macromolecules. Systems of primary
interest to us are protein-protein interactions involved in apoptosis
signaling, viral RNA translocation and viral budding pathways.
Publications
Selected publications from the last 5 years:
Sounier R, Blanchard L, Wu, Z., Boisbouvier J. (2006) "High-accuracy distance measurement between remote methyls in specifically protonated proteins." J Am Chem Soc 129(3), 472-3.
Wu, Z., Delaglio F, Wyatt K, Wistow G, Bax A. (2005) "Solution
structure of (gamma)S-crystallin by molecular fragment replacement
NMR.", Protein Sci. 14(12), 3101-14.
Wu, Z., Maderia M, Barchi JJ Jr, Marquez VE, Bax A. (2005) "Changes
in DNA bending induced by restricting nucleotide ring pucker studied
by weak alignment NMR spectroscopy.", Proc Natl Acad Sci U
S A. 102(1), 24-8.
O'Neil-Cabello, E.,Wu, Z., Bryce, D.L., Nikonowicz, E.P., and Bax,
A. (2004) " Enhanced spectral resolution in RNA HCP spectra for
measurement of (3)J(C2'P) and (3)J(C4'P) couplings and (31)P chemical
shift changes upon weak alignment." J Biomol NMR. 30(1),
61-70.
Boisbouvier, J., Wu, Z., Ono, A., Kainosho, M., and Bax, A. (2003) "Rotational
diffusion tensor of nucleic acids from 13C NMR relaxation." J
Biomol NMR. 27(2), 133-42.
Wu, Z., Delaglio, F., Tjandra, N., Zhurkin, V.B., and Bax, A. (2003) "Overall
structure and sugar dynamics of a DNA dodecamer from homo- and heteronuclear
dipolar couplings and 31P chemical shift anisotropy." J Biomol
NMR. 26(4), 297-315.
Lawrence, D.C., Stover, C.C., Noznitsky, J., Wu,
Z., and Summers, M.F.
(2003) "Structure of the intact stem and bulge of HIV-1 Psi-RNA
stem-loop SL1." J. Mol. Biol. 326(2), 529-542.
Wu, Z. and Bax, A. (2002) "Measurement of long-range 1H-1H dipolar
couplings in weakly aligned proteins." J. Am. Chem. Soc. 124(33),9672-9673.
Johnson, P.E., Turner, R.B., Wu, Z., Hairston, L., Guo, J., Levin,
J.G., and Summers, M.F. (2002) "A mechanism for plus-strand transfer
enhancement by the HIV-1 nucleocapsid protein during reverse transcription." Biochemistry
39(31), 9084-9091.
Wu, Z. and Bax, A. (2001) "Measurement of homonuclear proton couplings
based on cross-peak nulling in CT-COSY." J. Magn. Reson. 151(2), 242-252.
Wu, Z., Tjandra, N., and Bax, A. (2001) "31P chemical shift anisotropy
as an aid in determining nucleic acid structure in liquid crystals." J.
Am. Chem. Soc. 123(15), 3617-3618.
Wu, Z., Tjandra, N., and Bax, A. (2001) "Measurement of 1H3'-31P
dipolar couplings in a DNA oligonucleotide by constant-time NOESY difference
spectroscopy." J. Biomol. NMR. 19(4), 367-370.
Wu, Z., Ono, A., Kainosho, M., and Bax, A. (2001) "H...N hydrogen
bond lengths in double stranded DNA from internucleotide dipolar couplings." J.
Biomol. NMR. 19(4), 36136-5.
Delaglio, F., Wu, Z., and Bax, A. (2001) "Measurement of homonuclear
proton couplings from regular 2D COSY spectra." J. Magn. Reson.
149(2), 276-281.
